Fig. S2
Cardiovascular defects caused by morpholino-knockdown of ccm3a/b can be rescued by overexpression of ccm3b mRNA. (A) Light images of ccm3b mRNA injected and non-injected ccm3a/b morphant embryos at 54 hpf. The pronounced pericardial edema and circulatory block observed in 93% of the embryos upon 0.25 ng ccm3a/b morpholino injection (n = 83) are rescued by co-injection of 400 pg ccm3b mRNA. 63% of ccm3a/b morphant embryos injected with ccm3b mRNA had normal heart morphology and completely restored circulation (n = 76). (B) Confocal z-stack projections of dorsal head vasculature in ccm3a/b morphants indicate a decrease in the dilation and misconnections in cranial vessels upon ccm3b mRNA injection (yellow arrows).
Reprinted from Developmental Biology, 362(2), Yoruk, B., Gillers, B.S., Chi, N.C., and Scott, I.C., Ccm3 functions in a manner distinct from Ccm1 and Ccm2 in a zebrafish model of CCM vascular disease, 121-131, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.