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ZFIN ID: ZDB-FIG-120216-62
Yoruk et al., 2012 - Ccm3 functions in a manner distinct from Ccm1 and Ccm2 in a zebrafish model of CCM vascular disease. Developmental Biology   362(2):121-131 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: Long-pec
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Long-pec

Fig. 1

Morpholino knockdown of ccm3a/b in zebrafish embryos causes cardiovascular defects distinct from ccm1/2 mutants. (A) Light images of ccm3a/b morpholino injected embryos at 54 hpf. Morphant embryos display a severe pericardial edema and complete circulatory block, but no gross morphological defects when injected with 0.25–0.75 ng ccm3a/b ATG morpholino. (B) Fluorescent microscopy images of ccm3a/b morpholino injected and non-injected double transgenic kdrl:EGFP; gata1:dsRED embryos at 54 hpf. Morphants lack circulation with blood pooling at the yolk. (C) Confocal images of the ccm3a/b morpholino injected Tg(myl7:EGFP)twu34 wildtype and ccm2 (valentine) mutant embryos. At 54 hpf, ccm3a/b morphant hearts are dysmorphic but not enlarged, unlike the ccm2 (valentine) mutants whose hearts appear grossly dilated. (D) Confocal images of ccm3a/b morpholino injected and non-injected double transgenic kdrl:EGFP, myl7:ras-cherry embryos. Injection of morpholino causes thinning of the ventral aorta and the first aortic arches by 54 hpf.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
DsRed s843Tg; sd2Tg standard conditions Long-pec blood IFL
s843Tg; sd2Tg + MO1-pdcd10a,pdcd10b standard conditions Long-pec blood IFL
EGFP ccm2s259/s259; twu34Tg standard conditions Long-pec heart IFL
s843Tg; sd21Tg standard conditions Long-pec aortic arch IFL
Long-pec blood vessel endothelial cell IFL
Long-pec ventral aorta IFL
s843Tg; sd21Tg + MO1-pdcd10a,pdcd10b standard conditions Long-pec aortic arch IFL
Long-pec blood vessel endothelial cell IFL
Long-pec ventral aorta IFL
s843Tg; sd2Tg standard conditions Long-pec axial vasculature IFL
Long-pec blood vessel endothelial cell IFL
Long-pec cranial vasculature IFL
s843Tg; sd2Tg + MO1-pdcd10a,pdcd10b standard conditions Long-pec axial vasculature IFL
Long-pec blood vessel endothelial cell IFL
Long-pec cranial vasculature IFL
twu34Tg standard conditions Long-pec heart IFL
twu34Tg + MO1-pdcd10a,pdcd10b standard conditions Long-pec heart IFL
mCherry s843Tg; sd21Tg standard conditions Long-pec heart IFL
s843Tg; sd21Tg + MO1-pdcd10a,pdcd10b standard conditions Long-pec heart IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-pdcd10a,pdcd10b standard conditions Long-pec pericardium edematous, abnormal
Long-pec yolk ventral region edematous, abnormal
ccm2s259/s259; twu34Tg standard conditions Long-pec heart dilated, abnormal
s843Tg; sd21Tg + MO1-pdcd10a,pdcd10b standard conditions Long-pec aortic arch 1 decreased thickness, abnormal
Long-pec aortic arch 1 obstructed, abnormal
Long-pec blood circulation arrested, abnormal
Long-pec ventral aorta decreased thickness, abnormal
Long-pec ventral aorta obstructed, abnormal
s843Tg; sd2Tg + MO1-pdcd10a,pdcd10b standard conditions Long-pec blood circulation arrested, abnormal
Long-pec pericardium edematous, abnormal
Long-pec yolk ventral region edematous, abnormal
twu34Tg + MO1-pdcd10a,pdcd10b standard conditions Long-pec heart malformed, abnormal
Long-pec heart size, normal
Long-pec heart looping arrested, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 362(2), Yoruk, B., Gillers, B.S., Chi, N.C., and Scott, I.C., Ccm3 functions in a manner distinct from Ccm1 and Ccm2 in a zebrafish model of CCM vascular disease, 121-131, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.