FIGURE

Fig. 4

ID

ZDB-FIG-111223-24

Publication

Herwig et al., 2011 - Distinct Cellular Mechanisms of Blood Vessel Fusion in the Zebrafish Embryo

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Fig. 4

Visualization of Apical Membrane during Anastomosis

(A–A′′) Pdxl2 antibodies label apical cell membranes in ISVs and DLAV at 48 hpf.

(B–B′′) Pdxl2 is localized within a junctional ring (arrow) at the contact site of two tip cells at the onset of anastomosis (36 hpf).

(C–C′′) Apical membrane invaginating into a tip cell at 36 hpf is shown by Pdxl2 immunostaining.

(D–D′) In sih morphants at the same stage, the Pdxl2 staining is only seen within the junctional rings, indicating that apical membrane invagination does not take place in the absence of blood flow.

Also compare Movie S3 and Figure S3A. α-ZO1 is shown in white, α-Pdxl2 in green, and kdrl:EGFP in red. Scale bar in all pictures represents 20 μm.

Expression Data

Gene:	podxl2
Antibody:	Ab1-podxl2
Fish:	s843Tg
Anatomical Terms:	blood vessel endothelial cell apical plasma membrane dorsal longitudinal anastomotic vessel endothelial cell endothelial tip cell intersegmental vessel endothelial cell
Stage Range:	Prim-25 to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	nkuasrfp1aTg; ubs3Tg; ubs5Tg + MO1-tnnt2a
Knockdown Reagent:	MO1-tnnt2a
Observed In:	tube formation
Stage:	Prim-25

Phenotype Detail

Acknowledgments

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