IMAGE

Fig. 4

ID

ZDB-IMAGE-111223-24

Genes

podxl2

Antibodies

Ab1-podxl2

Source

Figures for Herwig et al., 2011

Image

Figure Caption

Fig. 4 Visualization of Apical Membrane during Anastomosis

(A–A′′) Pdxl2 antibodies label apical cell membranes in ISVs and DLAV at 48 hpf.

(B–B′′) Pdxl2 is localized within a junctional ring (arrow) at the contact site of two tip cells at the onset of anastomosis (36 hpf).

(C–C′′) Apical membrane invaginating into a tip cell at 36 hpf is shown by Pdxl2 immunostaining.

(D–D′) In sih morphants at the same stage, the Pdxl2 staining is only seen within the junctional rings, indicating that apical membrane invagination does not take place in the absence of blood flow.

Also compare Movie S3 and Figure S3A. α-ZO1 is shown in white, α-Pdxl2 in green, and kdrl:EGFP in red. Scale bar in all pictures represents 20 μm.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Curr. Biol.