FIGURE

Fig. 4

ID

ZDB-FIG-111201-57

Publication

Bachmann-Gagescu et al., 2011 - The Ciliopathy Gene cc2d2a controls Zebrafish Photoreceptor Outer Segment Development Through a Role in Rab8-dependent Vesicle Trafficking

Other Figures

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Fig. 4

cc2d2a is not required for ciliogenesis. (A and B) Connecting cilia (brackets) in wild-type (A) and cc2d2a-/- (B) photoreceptors demonstrated by transmission electron microscopy. (C and D) Apical view of ciliary axonemes labeled with acetylated tubulin antibodies (red) in wild-type (C) and cc2d2a-/- (D) whole-mount eyes in which cone outer segments are labeled with tg(TaCP:MCFP). (E and F) Peripheral views of the same eyes as in (C and D) showing only the acetylated tubulin staining. Note the shorter axonemes in cc2d2a-/- eyes (F) compared with wild-type (E). (G and H) Ciliary axonemes labeled with acetylated tubulin (red) and Ift88 (green) antibodies in wild-type (G) and cc2d2a-/- (H) retinal cryosections. Brackets highlight axonemal length. Embryos are 5 d.p.f. in (A–F) and 7 d.p.f. in (G and H). (I–N) Immunofluorescence with Arl13b antibody (red) highlighting cilia and with gamma-tubulin antibody (green) highlighting basal bodies in 24 h.p.f. whole-mount mz cc2d2a+/- (I, K and M) and mz cc2d2a-/- (J, L and N) embryos showing retinal neuronal progenitor cilia (arrowheads in insets of I and J), cerebellar neuronal progenitors (arrowheads in insets of K and L) and floorplate cilia (M and N). (O and P) Pronephric duct cilia stained with acetylated tubulin (red) and gamma-tubulin (green) antibodies in 48 h.p.f. whole-mount mz cc2d2a+/- (O) and mz cc2d2a-/- (P) embryos. The anterior–posterior axis is indicated by the arrows. (Q and R) Acetylated tubulin antibody staining of olfactory pit cilia at 3 d.p.f. in whole-mount wild-type (Q) and cc2d2a-/- (R) embryos. (I–L) and (Q and R) Single-confocal sections. (C–F) and (M–P) Projected stacks of confocal sections. Scale bars are 500 nm in (A and B), 5 µm in (C and D), 10 µm in (E and F), 4 µm in (G and H), 2 µm in (I–L), 10 µm in (M and N) and 5 µm in (O–R).

Expression Data

Genes:	arl13b CFP ift88
Antibodies:	Ab1-arl13b Ab1-tuba Ab1-tubg1
Fish:	WT cc2d2a^w38/w38 cc2d2a^w38/+ w62Tg cc2d2a^w38/+; w62Tg cc2d2a^w38/w38; w62Tg
Anatomical Terms:	cerebellum ciliary basal body cerebellum cilium eye photoreceptor cell axoneme eye photoreceptor cell ciliary basal body eye photoreceptor cell cilium floor plate ciliary basal body floor plate motile cilium olfactory pit non-motile cilium pronephric duct ciliary basal body pronephric duct motile cilium retinal cone cell photoreceptor outer segment membrane
Stage Range:	Prim-5 to Days 7-13

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	cc2d2a^w38/w38 cc2d2a^w38/w38 cc2d2a^w38/+ cc2d2a^w38/+ cc2d2a^w38/+; w62Tg cc2d2a^w38/w38; w62Tg
Observed In:	cerebellum cilium cilium assembly eye photoreceptor cell axoneme floor plate motile cilium olfactory pit non-motile cilium pronephric duct motile cilium
Stage Range:	Prim-5 to Days 7-13

Phenotype Detail

Acknowledgments

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