FIGURE

Fig. S3

ID

ZDB-FIG-111214-22

Publication

Bachmann-Gagescu et al., 2011 - The Ciliopathy Gene cc2d2a controls Zebrafish Photoreceptor Outer Segment Development Through a Role in Rab8-dependent Vesicle Trafficking

Other Figures

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Fig. S3

rab8 morphant phenotype and Rab8 localization at the base of the outer segment. (A-B) 4 dpf retinal cryosections of wild-type (A) and rab8 morphant (rab8MO)(B) embryos showing shortened outer segments stained with bodipy (red) and rhodopsin (4D2 antibody, green). (C-D) Higher power images of boxed areas in A and B with separate channel images for 4D2 (C′-D′) and bodipy (C′′-D′′) showing shortened and dysmorphic outer segments as well as rhodopsin mislocalization. (E) Rab8-cherry expression (red) in cryosections from wild-type TaCP:MCFP-expressing photoreceptors (outer segments are light blue) showing punctate localization at the base of the outer segment. Scale bars are 40 µm in A-B, 10 µm in C-D′′ and 4 µm in E. (F) Average outer segment length in cc2d2a -/- embryos injected with Rab8MO, injected with a control MO, uninjected and cc2d2a +/+ or +/- embryos injected with Rab8Mo. Scale bars are standard deviations. *** p<0.0001, NS non significant (p=0.7, Chi-square test). PR photoreceptors.

Expression Data

Gene:	rho
Antibody:	Ab5-rho
Fish:	WT WT + MO2-rab8a
Knockdown Reagent:	MO2-rab8a
Anatomical Terms:	retinal rod cell cytoplasm retinal rod cell photoreceptor outer segment
Stage:	Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO2-rab8a
Knockdown Reagent:	MO2-rab8a
Observed In:	photoreceptor cell outer segment organization retinal rod cell photoreceptor outer segment
Stage:	Day 4

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Hum. Mol. Genet.