Fig. 6

Nsf function is required in both pre- and postsynaptic cells to stabilize afferent innervation of hair cells.

A-E′′, Representative z-projections of a single neuromast from a top-down view (5 dpf). Wild-type (A), nsf^st53 mutants (B), and nsf^st53 mutants with Nsf-GFP expressed in hair cells (C), pLLG (D), or in both hair cells and pLLG (E) were labeled by antibodies against GFP (pseudo-colored light blue), MAGUK (green), and Ribeye b (red). Scale bar: 10 μm. F, The average number of MAGUK puncta per hair cell in wild-type (3.8 ±0.1, n = 7), nsf^st53 mutants (0.4±0.1, n = 7), and nsf^st53 mutants with hair-cell Nsf-GFP (1.2±0.4, n = 5), pLLG Nsf-GFP (2.2±0.2, n = 6), or both hair-cells and pLLG Nsf-GFP (3. 6±0.1, n = 7). G, Zebrafish larvae were stained with Zn12 and Ribeye b antibody. The fluorescent intensity (A.U.) of Zn12 was quantified in wild-type (1.813e6±147069, n = 6), nsf^st53 mutants (311769±43541, n = 6), and nsf^st53 mutants with Nsf-GFP rescued in hair cells (507787±97798, n = 5), pLLG (659674±85851, n = 5), or both hair cells and pLLG (1.335e6±223319, n = 8). The p-values were generated comparing the data from the nsf^st53 mutant to each transgenic mutant line.