Community Action Needed: Please respond to the NIH RFI
ZFIN ID: ZDB-FIG-111201-45
Mo et al., 2011 - Both pre- and postsynaptic activity of nsf prevents degeneration of hair-cell synapses. PLoS One   6(11):e27146 Full text @ PLoS One
Anatomical Terms:
Stage: Day 5

Fig. 2 Expression of Nsf in the CNS and lateral line organ (5 dpf).

A, An antibody against human NSF protein labels the larval nervous system (5 dpf). B, Labeling is absent in nsfst53 mutants. Images are projections of 5 optical sections of 10 μm thickness. Scale bar, 100 μm. C-F, Confocal projections (10×1 μm sections) of anti-NSF (red) and Ribeye b (Blue) in TgBAC(neurod:EGFP)nl1 transgenic fish. Nsf signal colocalized with anti-GFP (green) in the afferent nerve, and was also in Ribeye b-positive hair cells. Scale bar, 10 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP nl1Tg standard conditions Day 5 lateral line nerve IFL
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Qualifier Anatomy
Ab1-nsf IHC WT standard conditions Day 5 nervous system
IHC nl1Tg standard conditions Day 5 lateral line nerve
IHC Day 5 neuromast hair cell
IHC nsfast53/st53 standard conditions Day 5 Not Detected nervous system
Ab3-ctbp2l IHC nl1Tg standard conditions Day 5 neuromast hair cell
Phenotype Details No data available
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One