FIGURE

Fig. 5

ID

ZDB-FIG-111201-48

Publication

Mo et al., 2011 - Both pre- and postsynaptic activity of nsf prevents degeneration of hair-cell synapses

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Fig. 5

Apoptosis in nsf^st53 mutants (5 dpf).

Representative z-projections of the pLLG (thick dashes) and vagal ganglion (thin dashes) are shown in (A). In WT and nsf^st53 mutants, apoptotic cells (arrow heads) were labeled by Casp3 antibody (red), and neurons were labeled by acetylated Tubulin antibody (green). Scale bar: 20 μm. Images shown in all panels are 6 z-stacks of 1.5 µm. B, The percentage of apoptosis in the pLLG of both wild-type and nsf^st53 mutant larvae (in wild-type the percentage apoptosis are 1.4 ±0.9, n = 5, 0.0 ±0.0, n = 8 and 0.4 ±0.4, n = 8 from 3 dpf to 5 dpf respectively; 4.8 ±2.0, n = 7, 4.7 ±1.2, n = 7, and 5.8 ±1.4, n = 7 in nsf^st53 mutants). C, Cell numbers of the pLLG in wild-type increased from 25.6 ±1.6, n = 5 at 3 dpf to 29.6 ±1.5, n = 5 at 4 dpf and reached 37.0 ±2.3, n = 8 at 5 dpf, while the numbers changed from 22.3 ±0.9, n = 8 to 30.4 ±0.8, n = 8 and then to 25.9 ±2.2, n = 7 in nsf^st53 mutants. D, The ratio of the intensity of Zn12 labeling (Figure 3A) of nsf^st53 to wild-type labeling declined from 3 dpf to 5 dpf (76.2 ±9.5, n = 6, 58.2 ±4.1, n = 5, and 17.2 ±2.4, n = 7).

Expression Data

Antibodies:	Ab1-tuba Ab5-casp3
Fish:	WT nsfa^st53/st53
Anatomical Terms:	posterior lateral line ganglion vagal ganglion
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	nsfa^st53/st53
Observed In:	apoptotic process
Stage:	Day 5

Phenotype Detail

Acknowledgments

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