ZFIN ID: ZDB-FIG-111025-24
Madelaine et al., 2011 - Partially redundant proneural function reveals the importance of timing during zebrafish olfactory neurogenesis. Development (Cambridge, England)   138(21):4753-4762 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Prim-5 to Long-pec
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Prim-5 to Long-pec

Fig. 6

Neurod4 and Neurog1 act redundantly during OSN development in zebrafish. (A-D2) Confocal projections of in situ hybridisation and immunolabelling against deltaA and GFP in wild-type (A,A2), neurod4 morphant (B,B2), neurog1 mutant (C,C2) and neurog1/neurod4 double loss-of-function (D,D2) olfactory placodes of Tg(8.4neurog1:GFP) embryos at 24 hpf. Although no difference is apparent in the expression of deltaA in single loss-of-function embryos relative to wild-type siblings, deltaA expression is absent when both neurog1 and neurod4 function is abolished. (E-P) Confocal sections of in situ hybridisation labelling against olfactory marker protein (omp) in wild-type (E,I,M), neurod4 morphant (F,J,N), neurog1 mutant (G,K,O) and neurog1/neurod4 double loss-of-function (H,L,P) olfactory placodes at 24 (E-H), 36 (I-L) and 48 (M-P) hpf. Although omp expression resembles the wild-type pattern in the single loss-of-function context at 48 hpf, there is a delay in the initiation of omp expression in the absence of Neurog1 function. The expression of omp is never detected when both neurog1 and neurod4 function is abolished. Scale bars: 10 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
dla neurog1hi1059Tg/hi1059Tg; sb1Tg standard conditions Prim-5 olfactory placode sensory neuron ISH
neurog1hi1059Tg/hi1059Tg; sb1Tg + MO1-neurod4 standard conditions Prim-5 Not Detected olfactory placode sensory neuron ISH
sb1Tg standard conditions Prim-5 olfactory placode sensory neuron ISH
sb1Tg + MO1-neurod4 standard conditions Prim-5 olfactory placode sensory neuron ISH
GFP neurog1hi1059Tg/hi1059Tg; sb1Tg standard conditions Prim-5 olfactory placode pioneer neuron IHC
neurog1hi1059Tg/hi1059Tg; sb1Tg + MO1-neurod4 standard conditions Prim-5 olfactory placode pioneer neuron IHC
sb1Tg standard conditions Prim-5 olfactory placode pioneer neuron IHC
sb1Tg + MO1-neurod4 standard conditions Prim-5 olfactory placode pioneer neuron IHC
ompb neurog1hi1059Tg/hi1059Tg; sb1Tg standard conditions Prim-5 Not Detected olfactory placode sensory neuron ISH
Prim-25 peripheral olfactory organ olfactory receptor cell ISH
Long-pec peripheral olfactory organ olfactory receptor cell ISH
neurog1hi1059Tg/hi1059Tg; sb1Tg + MO1-neurod4 standard conditions Prim-5 Not Detected olfactory placode sensory neuron ISH
Prim-25 Not Detected peripheral olfactory organ olfactory receptor cell ISH
Long-pec Not Detected peripheral olfactory organ olfactory receptor cell ISH
sb1Tg standard conditions Prim-5 olfactory placode sensory neuron ISH
Prim-25 peripheral olfactory organ olfactory receptor cell ISH
Long-pec peripheral olfactory organ olfactory receptor cell ISH
sb1Tg + MO1-neurod4 standard conditions Prim-5 olfactory placode sensory neuron ISH
Prim-25 peripheral olfactory organ olfactory receptor cell ISH
Long-pec peripheral olfactory organ olfactory receptor cell ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
neurog1hi1059Tg/hi1059Tg; sb1Tg standard conditions Prim-5 olfactory placode pioneer neuron decreased amount, abnormal
Prim-25 olfactory placode pioneer neuron decreased amount, abnormal
Long-pec olfactory placode pioneer neuron decreased amount, abnormal
neurog1hi1059Tg/hi1059Tg; sb1Tg + MO1-neurod4 standard conditions Prim-5 olfactory placode pioneer neuron absent, abnormal
Prim-25 olfactory placode pioneer neuron absent, abnormal
Long-pec olfactory placode pioneer neuron absent, abnormal
sb1Tg + MO1-neurod4 standard conditions Prim-5 olfactory placode pioneer neuron decreased amount, abnormal
Prim-25 olfactory placode pioneer neuron decreased amount, abnormal
Long-pec olfactory placode pioneer neuron decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development