ZFIN ID: ZDB-FIG-111025-23
Madelaine et al., 2011 - Partially redundant proneural function reveals the importance of timing during zebrafish olfactory neurogenesis. Development (Cambridge, England)   138(21):4753-4762 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagent:
Anatomical Term:
Stage: Prim-5
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Prim-5

Fig. 5

Neurog1 and Neurod4 act redundantly during development of zebrafish early-born olfactory neurons. (A-D) Confocal projections of GFP from the Tg(8.4neurog1:GFP) transgene in the olfactory placode of wild-type (A), neurog1 mutant (B), neurod4 morphant (C) and neurog1/neurod4 double loss-of-function (D) embryos at 24 hpf. Abrogation of both Neurod4 and Neurog1 function leads to a severe deficit in the differentiation of early-born olfactory neurons. Placodes are oriented with anterior up. Scale bars: 10 μM. (E) Counts of early-born olfactory neurons per placode at 24 hpf in wild-type, neurog1 mutant, neurod4 morphant, neurog1/neurod4 double loss-of-function embryos and neurog1 mutant embryos in which Neurod4 has been mis-expressed. A minimum of twelve olfactory placode was analysed for each context. Error bars represent s.d. *P<0.05, **P<0.001, ***P<0.0005, determined by t-test.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
GFP neurog1hi1059Tg/hi1059Tg; sb1Tg standard conditions Prim-5 olfactory placode pioneer neuron IHC
neurog1hi1059Tg/hi1059Tg; sb1Tg + MO1-neurod4 standard conditions Prim-5 olfactory placode pioneer neuron IHC
sb1Tg standard conditions Prim-5 olfactory placode pioneer neuron IHC
sb1Tg + MO1-neurod4 standard conditions Prim-5 olfactory placode pioneer neuron IHC
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
neurog1hi1059Tg/hi1059Tg; sb1Tg standard conditions Prim-5 olfactory placode pioneer neuron decreased amount, abnormal
neurog1hi1059Tg/hi1059Tg; sb1Tg + MO1-neurod4 standard conditions Prim-5 olfactory placode pioneer neuron decreased amount, abnormal
sb1Tg + MO1-neurod4 standard conditions Prim-5 olfactory placode pioneer neuron decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development