FIGURE

Fig. S1

ID

ZDB-FIG-110812-42

Publication

Lim et al., 2011 - Motoneurons are essential for vascular pathfinding

Other Figures

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Fig. S1

netrin 1a and dcc morphants do not form a complete thoracic duct. (A,B,D,E) 5 dpf fli1a:egfp^y1 larvae; reversed contrast, confocal projections. (A2,B2,D2,E2) Enlargements of the boxed regions from A,B,D,E. (A-A2,D-D2) The thoracic duct (TD) forms normally (arrows) between the dorsal aorta (DA) and posterior cardinal vein (PCV) in uninjected control embryos. (B,B2,E,E2) The TD is absent in netrin1aSBMO or dccTBMO morphants (open arrowheads). (C,F) Quantitation of partial or complete TD formation in control embryos (77%), netrin1aSBMO morphants (25%) and dccTBMO morphants (45±19%; mean±s.e.m.); P-values, Mann-Whitney U test. n, number of hemisegments (number of embryos). PAC, parachordal chain; DA, dorsal aorta; PCV, posterior cardinal vein. Scale bars: 50 μm.

Expression Data

Gene:	EGFP
Fish:	y1Tg/+ y1Tg/+ + MO1-dcc y1Tg/+ + MO2-ntn1a
Knockdown Reagents:	MO1-dcc MO2-ntn1a
Anatomical Terms:	dorsal aorta parachordal vessel posterior cardinal vein thoracic duct
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	y1Tg/+ + MO1-dcc y1Tg/+ + MO2-ntn1a
Knockdown Reagents:	MO1-dcc MO2-ntn1a
Observed In:	lymph vessel development thoracic duct
Stage:	Day 5

Phenotype Detail

Acknowledgments

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