ZFIN ID: ZDB-FIG-110812-27
Naganawa et al., 2011 - Developmental transition of touch response from slow muscle-mediated coilings to fast muscle-mediated burst swimming in zebrafish. Developmental Biology   355(2):194-204 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibodies:
Fish:
Anatomical Terms:
Stage: Long-pec
PHENOTYPE:
Fish:
Observed In:
Stage: Long-pec

Fig. 5

Actin–myosin fibers are disorganized in flii fast muscles at 48 hpf. (A–D) The expression of FliI protein was assayed with anti-FliI at 48 hpf. FliI protein was expressed in wild-type slow muscle (A), mutant slow muscle (B) and wild-type fast muscle (C) but not in mutant fast muscle (D). (E–H) Double labeling with phalloidin (F-actin) and anti-myosin (slow muscle: F59; fast muscle: MF20) at 48 hpf. Co-labeling of F-actin and slow myosin was seen in wild-type (E) and mutant (F). F-actin was organized in wild-type fast muscle (G) but not in mutant fast muscle (H). (I–L) Electron micrographs of cross-sections of muscle at 48 hpf. Actin and myosin bundles were organized in wild-type slow muscle (I), mutant slow muscle (J) and wild-type fast muscle (K) but not in mutant fast muscle (L). Mitochondria are shaded blue.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
flii WT standard conditions Long-pec myotome fast muscle cell IHC
Long-pec myotome slow muscle cell IHC
fliimi372/mi372 standard conditions Long-pec Not Detected myotome fast muscle cell IHC
Long-pec myotome slow muscle cell IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab-F59 IHC WT standard conditions Long-pec myotome slow muscle cell
IHC fliimi372/mi372 standard conditions Long-pec myotome slow muscle cell
Ab-MF20 IHC WT standard conditions Long-pec myotome fast muscle cell
IHC fliimi372/mi372 standard conditions Long-pec myotome fast muscle cell
Phenotype Details
Fish Conditions Stage Phenotype
fliimi372/mi372 standard conditions Long-pec fast muscle cell myofibril disorganized, abnormal
Long-pec sarcomere organization process quality, abnormal
Long-pec slow muscle cell myofibril organized, normal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 355(2), Naganawa, Y., and Hirata, H., Developmental transition of touch response from slow muscle-mediated coilings to fast muscle-mediated burst swimming in zebrafish, 194-204, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.