ZFIN Image: Naganawa et al., 2011, Fig. 5

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Figure Caption

Fig. 5

Actin–myosin fibers are disorganized in flii fast muscles at 48 hpf. (A–D) The expression of FliI protein was assayed with anti-FliI at 48 hpf. FliI protein was expressed in wild-type slow muscle (A), mutant slow muscle (B) and wild-type fast muscle (C) but not in mutant fast muscle (D). (E–H) Double labeling with phalloidin (F-actin) and anti-myosin (slow muscle: F59; fast muscle: MF20) at 48 hpf. Co-labeling of F-actin and slow myosin was seen in wild-type (E) and mutant (F). F-actin was organized in wild-type fast muscle (G) but not in mutant fast muscle (H). (I–L) Electron micrographs of cross-sections of muscle at 48 hpf. Actin and myosin bundles were organized in wild-type slow muscle (I), mutant slow muscle (J) and wild-type fast muscle (K) but not in mutant fast muscle (L). Mitochondria are shaded blue.

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Acknowledgments

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Reprinted from Developmental Biology, 355(2), Naganawa, Y., and Hirata, H., Developmental transition of touch response from slow muscle-mediated coilings to fast muscle-mediated burst swimming in zebrafish, 194-204, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.