ZFIN ID: ZDB-FIG-110812-29
Naganawa et al., 2011 - Developmental transition of touch response from slow muscle-mediated coilings to fast muscle-mediated burst swimming in zebrafish. Developmental Biology   355(2):194-204 Full text @ Dev. Biol.
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Prim-5 to Long-pec

Fig. 7

Coilings and burst swimming are mediated by slow and fast muscles, respectively. (A–L) Superimposed movement of the trunk and tail. Control (A), ryr1b (E) and flii (I) morphants exhibited normal coilings at 24 hpf. smyhcs morphants (C), smyhcs-ryr1b (G) and smyhcs-flii (K) double morphants did not move in response to touch at 24 hpf. Control (B) and smyhcs (D) morphants showed normal burst swimming at 48 hpf. The burst swimming was compromised in ry1b (F) and flii (J) morphants at 48 hpf. smyhcs-ryr1b (H) and smyhcs-flii (L) double morphants did not move at 48 hpf. (M,N) Histograms showing the maximum headtail angles at 24 hpf (M) and 48 hpf (N).

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-flii standard conditions Prim-5 larval locomotory behavior process quality, normal
Prim-5 muscle contraction intensity, normal
Long-pec fast-twitch skeletal muscle fiber contraction process quality, abnormal
Long-pec larval locomotory behavior process quality, abnormal
Long-pec muscle contraction decreased intensity, abnormal
Long-pec myotome fast muscle cell decreased functionality, abnormal
WT + MO1-flii + MO1-smyhc + MO2-smyhc1 standard conditions Prim-5 fast-twitch skeletal muscle fiber contraction process quality, abnormal
Prim-5 larval locomotory behavior process quality, abnormal
Prim-5 muscle contraction decreased intensity, abnormal
Prim-5 myotome fast muscle cell decreased functionality, abnormal
Prim-5 myotome slow muscle cell decreased functionality, abnormal
Prim-5 slow-twitch skeletal muscle fiber contraction process quality, abnormal
Long-pec fast-twitch skeletal muscle fiber contraction process quality, abnormal
Long-pec myotome fast muscle cell decreased functionality, abnormal
Long-pec myotome slow muscle cell decreased functionality, abnormal
Long-pec slow-twitch skeletal muscle fiber contraction process quality, abnormal
WT + MO1-smyhc + MO2-smyhc1 standard conditions Prim-5 larval locomotory behavior process quality, abnormal
Prim-5 muscle contraction decreased intensity, abnormal
Prim-5 myotome slow muscle cell decreased functionality, abnormal
Prim-5 slow-twitch skeletal muscle fiber contraction process quality, abnormal
Long-pec larval locomotory behavior process quality, normal
Long-pec myotome slow muscle cell decreased functionality, abnormal
Long-pec slow-twitch skeletal muscle fiber contraction process quality, abnormal
WT + MO1-smyhc + MO2-smyhc1 + MO4-ryr1b standard conditions Prim-5 fast-twitch skeletal muscle fiber contraction process quality, abnormal
Prim-5 larval locomotory behavior process quality, abnormal
Prim-5 muscle contraction decreased intensity, abnormal
Prim-5 myotome fast muscle cell decreased functionality, abnormal
Prim-5 myotome slow muscle cell decreased functionality, abnormal
Prim-5 slow-twitch skeletal muscle fiber contraction process quality, abnormal
Long-pec fast-twitch skeletal muscle fiber contraction process quality, abnormal
Long-pec larval locomotory behavior process quality, abnormal
Long-pec muscle contraction decreased intensity, abnormal
Long-pec myotome fast muscle cell decreased functionality, abnormal
Long-pec myotome slow muscle cell decreased functionality, abnormal
Long-pec slow-twitch skeletal muscle fiber contraction process quality, abnormal
WT + MO4-ryr1b standard conditions Prim-5 fast-twitch skeletal muscle fiber contraction process quality, abnormal
Prim-5 larval locomotory behavior process quality, normal
Prim-5 muscle contraction intensity, normal
Prim-5 myotome fast muscle cell decreased functionality, abnormal
Long-pec fast-twitch skeletal muscle fiber contraction process quality, abnormal
Long-pec larval locomotory behavior process quality, abnormal
Long-pec muscle contraction decreased intensity, abnormal
Long-pec myotome fast muscle cell decreased functionality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 355(2), Naganawa, Y., and Hirata, H., Developmental transition of touch response from slow muscle-mediated coilings to fast muscle-mediated burst swimming in zebrafish, 194-204, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.