ZFIN ID: ZDB-FIG-110720-8
Anderson et al., 2011 - The L6 domain tetraspanin Tm4sf4 regulates endocrine pancreas differentiation and directed cell migration. Development (Cambridge, England)   138(15):3213-3224 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: 20-25 somites to Long-pec
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: 20-25 somites to Long-pec

Fig. 4

tm4sf4 inhibits α and β cell specification, and is necessary for µ cell fates. (A-P) Hormone mRNA levels were measured by qRT-PCR comparing uninjected (blue) and tm4sf4 morphant (red) zebrafish embryos: insulin (A), glucagon (D), ghrelin (K), somatostatin (N). Hormone mRNA expression pattern was determined by in situ hybridization comparing uninjected and tm4sf4 morphant embryos at 20.5 hpf for insulin (B,C) and glucagon (E-J), and at 48 hpf for ghrelin (L,M) and somatostatin (O,P). All images were taken at 40× magnification except G,H taken at 10×; yellow boxes represent areas enlarged in I and J. (Q) Knockdown efficiency of Tm4splMO was determined at all stages. Error bars represent s.e.m. *P<0.05.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
gcga WT standard conditions 20-25 somites pancreatic A cell ISH
WT + MO2-tm4sf4 standard conditions 20-25 somites pancreatic A cell ISH
ghrl WT standard conditions Long-pec pancreatic epsilon cell ISH
WT + MO2-tm4sf4 standard conditions Long-pec pancreatic epsilon cell ISH
ins WT standard conditions 20-25 somites pancreatic B cell ISH
WT + MO2-tm4sf4 standard conditions 20-25 somites pancreatic B cell ISH
sst1.1 WT standard conditions Long-pec somatostatin secreting cell ISH
WT + MO2-tm4sf4 standard conditions Long-pec somatostatin secreting cell ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO2-tm4sf4 standard conditions 20-25 somites endocrine pancreas development disrupted, abnormal
20-25 somites pancreatic A cell increased amount, abnormal
20-25 somites pancreatic B cell increased amount, abnormal
Long-pec endocrine pancreas development disrupted, abnormal
Long-pec pancreatic A cell amount, normal
Long-pec pancreatic epsilon cell decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development