ZFIN ID: ZDB-FIG-110720-7
Anderson et al., 2011 - The L6 domain tetraspanin Tm4sf4 regulates endocrine pancreas differentiation and directed cell migration. Development (Cambridge, England)   138(15):3213-3224 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Dome to Days 7-13

Fig. 3

The tm4sf4 gene and regulation by nkx2.2a is conserved in zebrafish. (A) CLUSTAL W species alignment between human, mouse and zebrafish Tm4sf4. Identical (*), highly conserved (:) and weakly conserved (.) amino acids are indicated. Small/hydrophobic residues (red), acidic residues (blue), basic residues (magenta), hydroxyl/amine/basic residues (green) are shown. (B,C) Temporal qRT-PCR tm4sf4 mRNA analysis of early (B) and late (C) stage zebrafish embryos. (D-F) tm4sf4 in situ hybridization on wild-type (D,E) and nkx2.2a morphant (F) embryos. In, intestine; L, liver; P, pancreas. Images taken at 20× magnification. (G-I) qRT-PCR comparing uninjected (blue) and nkx2.2a morphant (red) embryo expression of tm4sf4 (G), insulin (H) and ghrelin (I). Error bars represent s.e.m.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
ghrl WT standard conditions 20-25 somites to Long-pec whole organism RTPCR
WT + MO3-nkx2.2a standard conditions 20-25 somites to Long-pec whole organism RTPCR
ins WT standard conditions 20-25 somites to Long-pec whole organism RTPCR
WT + MO3-nkx2.2a standard conditions 20-25 somites to Long-pec whole organism RTPCR
tm4sf4 WT standard conditions Dome to 50%-epiboly Not Detected whole organism RTPCR
1-4 somites whole organism RTPCR
10-13 somites whole organism RTPCR
14-19 somites whole organism RTPCR
20-25 somites whole organism RTPCR
20-25 somites to Long-pec whole organism RTPCR
Prim-5 whole organism RTPCR
Prim-15 whole organism RTPCR
Prim-25 pancreatic bud ISH
Long-pec intestine ISH
Long-pec liver ISH
Long-pec pancreas ISH
Long-pec whole organism RTPCR
Pec-fin whole organism RTPCR
Protruding-mouth whole organism RTPCR
Day 6 whole organism RTPCR
Days 7-13 whole organism RTPCR
WT + MO3-nkx2.2a standard conditions 20-25 somites to Long-pec whole organism RTPCR
Prim-25 pancreatic bud ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development