ZFIN ID: ZDB-FIG-110429-13
Fujita et al., 2011 - Assembly and patterning of the vascular network of the vertebrate hindbrain. Development (Cambridge, England)   138(9):1705-1715 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Prim-15 to Pec-fin
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Prim-15 to Pec-fin

Fig. 3

Central artery formation and patterning. (A,B) Single horizontal section through the hindbrains of 54 (A) and 60 (B) hpf Tg(kdrl:GFP) embryos. The CtA in the third rhombomere (r3) links directly to the BA from early stages (red arrows in A), whereas CtAs in r4 and r5 do not initially connect to the BA. One or two direct connections are also usually present in r6 or r7 (yellow arrow in A) by this stage. Direct connections in r4 and r5 are formed later (blue arrows in B). Each stem of the CtAs in r3 to r6 is indicated by a purple arrowhead. (C,D) CtAs avoid Olig2+ clusters in r5 and r6. Dorsal (C) and lateral (D) view of confocal images in a 48 hpf Tg(fli1a:EGFP)y1; Tg(olig2:DsRed2)vu19 double-transgenic embryo, showing DsRed2-positive neuroepithelial precursors (red) and EGFP-positive blood vessels (green). The CtAs in r5 and r6 (arrows) appear to turn away from and avoid the nearby Olig2+ clusters. (E-H) CtAs are patterned normally in tnnt2a MO-injected ‘silent heart’ animals that lack blood circulation. Confocal images of the hindbrain vasculature in 34 (E,F) and 60 (G,H) hpf Tg(fli1a:EGFP)y1 control (E,G) or tnnt2a (F,H) MO-injected zebrafish embryos. BA, red arrowheads; CtAs, purple arrowheads. Dorsal view, rostral to the left. Scale bars: 50 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
DsRed2 vu19Tg; y1Tg standard conditions Long-pec rhombomere 5 ISH
Long-pec rhombomere 6 ISH
Pec-fin rhombomere 5 ISH
Pec-fin rhombomere 6 ISH
EGFP vu19Tg; y1Tg standard conditions Long-pec basilar artery ISH
Long-pec primordial hindbrain channel ISH
Long-pec rhombomere 3 central artery ISH
Pec-fin rhombomere 3 central artery ISH
Pec-fin rhombomere 4 central artery ISH
Pec-fin rhombomere 5 central artery ISH
Pec-fin rhombomere 6 central artery ISH
y1Tg control Prim-15 basilar artery IFL
Prim-15 primordial hindbrain channel IFL
Prim-15 primordial hindbrain channel angiogenic sprout IFL
Pec-fin basilar artery IFL
Pec-fin central artery IFL
Pec-fin primordial hindbrain channel IFL
y1Tg + MO1-tnnt2a standard conditions Prim-15 basilar artery IFL
Prim-15 primordial hindbrain channel IFL
Prim-15 primordial hindbrain channel angiogenic sprout IFL
Pec-fin basilar artery IFL
Pec-fin central artery IFL
Pec-fin primordial hindbrain channel IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
y1Tg + MO1-tnnt2a standard conditions Prim-15 basilar artery decreased diameter, abnormal
Prim-15 basilar artery structure, normal
Prim-15 blood circulation absent, abnormal
Pec-fin basilar artery decreased diameter, abnormal
Pec-fin basilar artery structure, normal
Pec-fin blood circulation absent, abnormal
Pec-fin central artery decreased diameter, abnormal
Pec-fin central artery structure, normal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development