FIGURE

Fig. S9

ID

ZDB-FIG-110429-27

Publication

Fujita et al., 2011 - Assembly and patterning of the vascular network of the vertebrate hindbrain

Other Figures

All Figure Page

Back to All Figure Page

Fig. S9

PI3K signaling is required for HUVEC migration in vitro and for PHBC migration in vivo. (A) SDF-1α-induced dose-dependent migration of HUVECs after a 5 hour incubation, measuring the number of transwell-migrated cells. (B) HUVEC transwell migration in response to 100 ng/ml SDF-1α (column 2) and after pretreatment with either 1 mM AMD3100 (column 4) or 10 mM LY294002 (column 6). Shown (A,B) are the mean (with s.e.m.) obtained by measuring eight randomly selected fields of transwell-migrated cells. (C,D) Confocal images of PHBCs and BA in the hindbrain of 32 hpf Tg(fli1a:EGFP)^y1 animals treated from 24-32 hpf with either control DMSO carrier (C) or the PI3K inhibitor LY294002 (D). Dorsal view, rostral to the left. Scale bar: 50 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development