Fig. s3
GFP expression in the hearts of Tg(wt1b:GFP) zebrafish mirrors cryoinjury-induced upregulation of wt1b mRNA in the epicardium. In situ hybridization on adjacent sections of cryoinjured zebrafish hearts at 3 dpi. (A-C) Sections were hybridized with wt1a (A), wt1b (B) and gfp (C) riboprobes. (A2-C2) Higher magnification views of boxed areas. Arrowheads indicate stained epicardial cells. wt1a mRNA is expressed in a subset of epicardial cells. wt1b is expressed in a larger population of epicardial cells. gfp expression overlaps with the expression domain of wt1b. IA, injured area; dpi, days postinjury; V, ventricle. |