ZFIN ID: ZDB-FIG-110322-32
Pikulkaew et al., 2011 - The knockdown of maternal glucocorticoid receptor mRNA alters embryo development in zebrafish. Developmental dynamics : an official publication of the American Association of Anatomists   240(4):874-889 Full text @ Dev. Dyn.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Long-pec to Day 5
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Prim-5 to Day 6

Fig. 4

A:grATG1MO-injected embryos failed to inflate the swim bladder (red arrowhead) and displayed a voluminous yolk sac and yolk extension (small arrow), pericardial oedema (asterisk), disrupted myoseptal melanophores (red arrow), and less developed trunk fin (black arrowhead) compared with grmismMO control at 5 dpf. B: Left: alkaline phosphatase staining showing reduced or absent sub-intestinal veins (SIV, arrow) in grATGMO embryos as compared to well-organized SIV in WT embryos at 3 dpf; results were the same using the Tg(fli1a:EGFP)y1 zebrafish transgenic line (right). C: Histology of zebrafish grATG1MO and WT embryos at 6 (sagital sections) and 5 (trasversal sections) dpf. Ov, otic vesicle; Li, liver; In, intestine; Yo, yolk sac; Sb, swim bladder. D: Top: expression of mnx1 in grATG1MO and WT embryos as detected by WISH. Lateral view shows mnx1 expression in the swim bladder epithelium (sb) at 48 hpf and 5 dpf. Other domains of expression are rhombomeres 5 and 6 (r-5,6), neural tube (nt), and endocrine pancreas (p). Bottom: Effects of grATG1MO and grATG2MO, grmismMO, and grsplicMO on swim bladder inflation in larvae at 5 dpf. Results show the percentage average (means ± SD) of individual larvae (n = 20; in triplicate) that developed the gas bladder. Means with asterisks indicate statistically significant differences from controls (P < 0.05). E: TUNEL analysis to detect apoptotic nuclei in WT, grATG1MO grmismMO and grsplicMO embryos at 10 and 24 hpf.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
mnx1 WT standard conditions Long-pec brain ISH
Long-pec endocrine pancreas ISH
Long-pec rhombomere 5 ISH
Long-pec rhombomere 6 ISH
Long-pec swim bladder bud epithelium ISH
Day 5 swim bladder anatomical margin ISH
WT + MO2-nr3c1 standard conditions Long-pec brain ISH
Long-pec endocrine pancreas ISH
Long-pec rhombomere 5 ISH
Long-pec rhombomere 6 ISH
Long-pec swim bladder bud epithelium ISH
Day 5 swim bladder ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO2-nr3c1 standard conditions Prim-5 apoptotic process increased occurrence, abnormal
Day 5 ball edematous, abnormal
Day 5 extension edematous, abnormal
Day 5 pericardium edematous, abnormal
Day 5 swim bladder non-functional, abnormal
Day 6 intestine decreased width, abnormal
Day 6 liver decreased mass, abnormal
Day 6 myoseptum melanocyte disorganized, abnormal
Day 6 post-vent region kinked, abnormal
y1Tg + MO2-nr3c1 standard conditions Long-pec subintestinal vein absent, abnormal
Long-pec subintestinal vein decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental dynamics : an official publication of the American Association of Anatomists for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dev. Dyn.