ZFIN ID: ZDB-FIG-110318-24
Faucherre et al., 2011 - Delaying gal4-driven gene expression in the zebrafish with morpholinos and gal80. PLoS One   6(1):e16587 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Terms:
Stage Range: Prim-5 to Day 6

Fig. 4 Gal4 morpholino temporarily inhibits Gal4 expression in Gal4-VP16 lines.

(A) Embryos resulting from a cross between Tg[hsp70:Gal4] and Tg[UAS:mem-TdTomato] fish were either non-injected (NI, Aa-b, N = 27) or injected with 3 ng of Gal4 MO (Ac-d, N = 37). Representative specimens are depicted at 2 dpf. (B) Embryos resulting from a cross of Tg[ET(hsp:Gal4VP16s1006t);UAS:Kaede] double transgenic animals were either non-injected (NI, Ba-d, N = 47) or injected with 3 ng of Gal4 MO (Be-h, N = 52). Representative specimens are depicted at 24 hpf (Ba, e), 4 dpf (Bb, f) and 6 dpf (Bc-d, Bg-h). (C) Embryos resulting from a cross of Tg[hspGFF53A;UAS:EGFP] double transgenic animals were either non-injected (NI, Ca-b, N = 64) or injected with 3 ng of Gal4 MO (Cc-d, N = 58). Representative specimens are depicted at 48 hpf (Ca, c) and 4 dpf (Cb, d). (D). Embryos resulting from a cross of Tg[hspGFF53A;UAS:EGFP] double transgenic animals were either non-injected (NI, Da), injected with 5 ng of Gal4 MO (Db) or injected with 5 ng of a control MO (Dc). Representative specimens are depicted at 48 hpf. (Scale bars: 600 μm for A; 300 μm for B and C, 150 μm for D).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP nkhspGFF53AEt; nkuasgfp1aTg heat shock Long-pec inner ear afferent neuron IFL
Long-pec lateral line afferent neuron IFL
Long-pec muscle IFL
Day 4 inner ear afferent neuron IFL
Day 4 lateral line afferent neuron IFL
Day 4 muscle IFL
Kaede s1006tEt; s1999tTg heat shock Prim-5 extraocular musculature IFL
Prim-5 lateral line ganglion afferent neuron IFL
Prim-5 trunk musculature IFL
Day 4 extraocular musculature IFL
Day 4 lateral line ganglion afferent neuron IFL
Day 4 trunk musculature IFL
Day 6 extraocular musculature IFL
Day 6 lateral line ganglion afferent neuron IFL
Day 6 trunk musculature IFL
Tomato bc2Tg; kca4Tg heat shock Long-pec whole organism IFL
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One