Gal80 expression inhibits Gal4 activity.
(A–H) Embryos resulting from a cross between Tg[hsp70:Gal4] and Tg[UAS:Kaede] fish were either non-injected (NI, A–D) or injected with 100 pg of mRNA encoding full-length Gal80 (E–H). Representative specimens are depicted at 24 hpf (A–B, N = 18 GFP+/72 fish; E–F, N = 0 GFP+/75 fish) and 5 dpf (C–D, N = 18 GFP+/77 fish; G–H, N = 19 GFP+/82 fish). In H, asterisks indicate the fish displaying GFP. (I–N) Embryos resulting from a cross of Tg[UAS:Kaede] fish were injected with a DNA encoding full-length Gal4 under the control of the HuC promoter either alone (NI, I–K, N = 32) or with 100 pg of mRNA encoding full-length Gal80 (L–N, N = 37). Representative specimens are depicted at 48 hpf, 3 and 7 dpf.