ZFIN ID: ZDB-FIG-110128-32
Xu et al., 2010 - Setdb2 restricts dorsal organizer territory and regulates left-right asymmetry through suppressing fgf8 activity. Proceedings of the National Academy of Sciences of the United States of America   107(6):2521-2526 Full text @ Proc. Natl. Acad. Sci. USA
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: 20-25 somites to Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: 20-25 somites to Protruding-mouth

Fig. S4 Organ laterality is randomized in setdb2-knockdown embryos with MO1. (A–D) Whole-mount mRNA in situ hybridization of visceral and diencephalic laterality in the setdb2 MO1-injected embryos at the indicated developmental stages with probes cmlc2 (A), lefty1 (B), cebpα (C, arrows), and insulin (D). (E) Determination of pancreatic laterality in 72-hpf Tg(insulin:EGFP) transgenic embryos injected with setdb2 MO1. Dashed lines denote the embryonic midline. LV, liver; G, gut. All embryos are dorsal views with head to the top.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
cebpa WT + MO1-setdb2 standard conditions Protruding-mouth gut ISH
Protruding-mouth liver ISH
EGFP rj29Tg + MO1-setdb2 standard conditions Protruding-mouth pancreas IFL
ins WT + MO1-setdb2 standard conditions Protruding-mouth pancreas ISH
lft1 WT + MO1-setdb2 standard conditions 20-25 somites diencephalon ISH
myl7 WT + MO1-setdb2 standard conditions Prim-15 heart tube ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-setdb2 standard conditions 20-25 somites determination of left/right asymmetry in diencephalon disrupted, abnormal
20-25 somites diencephalon structure, abnormal
Prim-15 determination of heart left/right asymmetry disrupted, abnormal
Prim-15 heart tube centered, abnormal
Prim-15 heart tube mislocalised radially, abnormal
Protruding-mouth determination of digestive tract left/right asymmetry disrupted, abnormal
Protruding-mouth determination of liver left/right asymmetry disrupted, abnormal
Protruding-mouth determination of pancreatic left/right asymmetry disrupted, abnormal
Protruding-mouth gut centered, abnormal
Protruding-mouth gut mislocalised radially, abnormal
Protruding-mouth liver centered, abnormal
Protruding-mouth liver mislocalised radially, abnormal
Protruding-mouth pancreas centered, abnormal
Protruding-mouth pancreas mislocalised radially, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA