ZFIN ID: ZDB-FIG-101103-28
Lopes et al., 2010 - Notch signalling regulates left-right asymmetry through ciliary length control. Development (Cambridge, England)   137(21):3625-3632 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Term:
Stage: Bud

Fig. 3 DeltaD modulates foxj1a expression. (A,B) Expression pattern of sox17 in Kupffer′s vesicle precursor cells at bud stage in wild-type (A) and aei-/- mutant (B) embryos. (C-F) Expression pattern of foxj1a in Kupffer′s vesicle precursor cells at bud stage in a wild-type (C), an aei-/- mutant (D), a wild type overexpressing deltaD and a wild type overexpressing NICD (F). (G,H) Foxj1a mRNA injection increases ciliary length. (G) Tail region of an aei-/- mutant embryo showing GFP-positive cells that were co-targeted with foxj1a and GFP mRNA. In these cells, cilia are much larger than in adjacent non-targeted cells. (H) Cells from the Kupffer′s vesicle in an aei-/- mutant embryo that was co-injected with foxj1a and GFP mRNA showing rescued motile cilia judged by the increased ciliary length. α-Acetylated tubulin labels cilia in red, DAPI labels DNA in blue and GFP labels targeted cells in green.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
foxj1a WT standard conditions Bud forerunner cell group ISH
dldtr233/tr233 standard conditions Bud forerunner cell group ISH
sox17 WT standard conditions Bud forerunner cell group ISH
dldtr233/tr233 standard conditions Bud forerunner cell group ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development