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ZFIN ID: ZDB-FIG-101103-27
Lopes et al., 2010 - Notch signalling regulates left-right asymmetry through ciliary length control. Development (Cambridge, England)   137(21):3625-3632 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibodies:
Fish:
Anatomical Terms:
Stage: 10-13 somites
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: 10-13 somites

Fig. 2

DeltaD regulates cilia length in the Kupffer′s vesicle.(A,D,G) ZO-1 immunostaining of a representative wild-type embryo (A), an aei-/- mutant embryo (D) and a deltaD-overexpressing embryo (G). (B,E,H) aPKC immunostaining of a representative wild-type embryo (B), an aei-/- mutant embryo (E) and a deltaD-overexpressing embryo (H). (C,F,I) DIC images of live embryos showing a wild-type KV (C), a KV from an aei-/- embryo (F) and a KV from a deltaD-overexpressing embryo (I). (J) Quantification of ciliary length at 10-somite stages in wild type, in aei-/- mutants and in embryos where Notch signalling was hyperactivated by overexpressing NICD (NICD-OE) or deltaD (deltaD-OE). Asterisks indicate experimental conditions that produce changes that are significantly different from wild type (P<0.001, Student′s t-test, two tail for two samples assuming unequal variances). (K) Histogram showing the distribution of Kupffer′s vesicle ciliary length at 10-somite stages in aei-/- mutant embryos (black bars), wild-type embryos (black line) and embryos subjected to deltaD-OE (grey bars). (L-N) Confocal images of all z-sections spaced 1 ┬Ám apart through the entire ciliated Kupffer′s vesicle at the 10-somite stage. Cilia are labelled with anti-acetylated α-tubulin in a wild-type embryo (L), an aei-/- mutant (M) and an embryo subjected to deltaD-OE (N). (O,P) Wild-type deltaD expression at the 10-somite stage in whole mount (O) and in resin section (P). The white and the black asterisks label the position and the lumen of the Kupffer′s vesicle, respectively. (Q,R) deltaD-MO co-injected with fluorescein lineage tracer in the dorsal forerunner cells (DFCs). (Q) Injected embryo with non-targeted Kupffer′s vesicle cells showing normal length cilia. (R) Fluorescein-positive Kupffer′s vesicle cells with shorter cilia. Error bars indicate s.d.m.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
dld WT standard conditions 10-13 somites Kupffer's vesicle ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-prkcz IHC WT standard conditions 10-13 somites Kupffer's vesicle epithelial cell
IHC dldtr233/tr233 standard conditions 10-13 somites Kupffer's vesicle epithelial cell
Ab1-tjp1 IHC WT standard conditions 10-13 somites Kupffer's vesicle bicellular tight junction
IHC dldtr233/tr233 standard conditions 10-13 somites Kupffer's vesicle bicellular tight junction
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO2-dld standard conditions 10-13 somites Kupffer's vesicle motile cilium decreased length, abnormal
dldtr233/tr233 standard conditions 10-13 somites Kupffer's vesicle morphology, normal
10-13 somites Kupffer's vesicle motile cilium decreased length, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development