Fig. S7
PCR analysis for GFP detection using primers located in the YFP gene, 2% agarose gel containing ethidium bromide, showing the detection of a faint background band. A. vasa::egfp testes were used as a positive control. Transplanted male after 3 weeks of transplantation, transplanted female after 1 month of transplantation, H2O water as negative control. Bands at left side are DNA markers from SMART ladder (Eurogentec). B. PCR detection using primers located in the PD-ECGF (plated-derived endothelial cell growth factor) gene as positive control for genomic DNA in the different individuals, 2% agarose gel containing ethidium bromide, showing the detection of a faint background band. Bands at left side are DNA markers from SMART ladder. |