Fig. S5
Preparation of female zebrafish recipients for SSC transplantation. A?C. Ovaries histological sections of ovoposition-induced females stained with PAS (Periodic acid Schiff). Arrows indicate postovulatory follicles after ovoposition. Note in C the available follicle cells which can support the transplanted SSC development. Scales bars = 250 μm (A), 100 μm (B), 25 μm (C). D. Number of postovulatory follicles (POFs)/mm2 of ovary in control (n = 5) and ovopositioned females (n = 5). Bars represent the mean± SE. * means significant differences (p<0.05) between the groups (Student unpaired t-test). |