FIGURE

Fig. 2

ID

ZDB-FIG-101012-40

Publication

Nóbrega et al., 2010 - Spermatogonial stem cell niche and spermatogonial stem cell transplantation in zebrafish

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Fig. 2

Label retaining cell (LRC) approach in zebrafish testes.

A. BrdU labeling index of type Aund* and Aund during BrdU exposure (4, 10, and 30 h) and after 4, 11, 18 and 25 days of chase. Bars represent the percentage mean ± SE (n = 5); different letters denote significant differences (p<0.05) in time. B. Distribution of BrdU-positive Aund* and Aund spermatogonia after 18 and 25 days of chase. Bars represent the mean ± SE which are expressed as percentage, and different letters indicate significant differences (p<0.05) among the groups. C–H. BrdU immunodetection with PAS staining after 4 (C–E), 11 (F), 18 (G), and 25 (H) days of chase. Note that BrdU immunostaining is diluted as a consequence of the progression of spermatogenesis. Only the slow-cycling cells (stem cells candidates) are able to retain the BrdU label for long periods of time. LRCs are indicated by arrows. Some of LRC are near to blood vessels (arrowhead) (E). Insets are high magnification of the LRCs (type A undifferentiated spermatogonia). The interstitium is delimited by spotted lines. Scale bars = 10 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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