FIGURE

Fig. 8

ID

ZDB-FIG-100903-48

Publication

Wang et al., 2010 - Moesin1 and Ve-cadherin are required in endothelial cells during in vivo tubulogenesis

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Fig. 8

Knockdown of Ve-cadherin impairs lumen formation. (A-F) Confocal images of ISVs in Tg(fli1:egfp)^y1 zebrafish embryos probed for Ve-cadherin (red) at 54 hpf. Ve-cadherin labeling (arrows) is observed in control embryos (A-C), but not in Ve-cadherin knockdown embryos (D-F). (G-L) Confocal images of ISVs in Tg(fli1:egfp)^y1 embryos labeled with ZO-1 antibody (red) at 54 hpf. ZO-1-associated junctions (arrows) are observed in control embryos (G-I), but are not detected in some regions of the ISV (arrowhead) in a Ve-cadherin knockdown embryo (J-L). (M-P) Confocal images of ISVs in Tg(flk1:moesin1-egfp) embryos at 32 hpf. The primary lumen (arrow) is forming in a control embryo (M). Putative vacuoles or intercellular spaces (arrows in the higher magnification images O and P) are observed throughout the cytoplasm in endothelial cells in Ve-cadherin knockdown embryos (N-P), without formation of primary lumen (N, arrowhead).

Expression Data

Genes:	cdh5 EGFP
Antibodies:	Ab1-cdh5 Ab1-tjp1
Fish:	is1Tg y1Tg is1Tg + MO6-cdh5 y1Tg + MO6-cdh5
Knockdown Reagent:	MO6-cdh5
Anatomical Terms:	blood vessel endothelial cell adherens junction blood vessel endothelial cell bicellular tight junction intersegmental vessel blood vessel endothelial cell
Stage Range:	Prim-15 to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	is1Tg + MO6-cdh5 y1Tg + MO6-cdh5
Knockdown Reagent:	MO6-cdh5
Observed In:	blood vessel lumenization intersegmental vessel
Stage Range:	Prim-15 to Long-pec

Phenotype Detail

Acknowledgments

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