FIGURE

Fig. 6

ID

ZDB-FIG-100903-46

Publication

Wang et al., 2010 - Moesin1 and Ve-cadherin are required in endothelial cells during in vivo tubulogenesis

Other Figures

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Fig. 6

Moesin1 is required during ISVs tubulogenesis. (A-D) Confocal images of ISVs from Tg(flk1:mCherry-β-actin) living zebrafish embryos. (A,C) Embryos injected with 4 ng control MO. The lumen (arrows) is seen at 30 hpf (A) and is well formed at 54 hpf (C). (B,D) Embryos injected with 4 ng moesin1 MO. The primary lumen is not observed at 32 hpf (B), nor at 54 hpf (D), although a few putative vacuoles or intercellular spaces are observed (B, arrowheads). (E,F) Confocal time-lapse images of wild-type and Moesin1 knockdown Tg(flk1:mCherry-β-actin) embryos. The time format is minutes:seconds. (E) In wild-type embryos, formation of the primary lumen is observed (arrows) at 30 hpf. (F) In Moesin1 knockdown embryos, mCherry-β-Actin remains throughout the cytoplasm and the primary lumen is not observed at 33 hpf. n, nucleus.

Expression Data

Gene:	mCherry
Fish:	is2Tg is2Tg + MO1-msna
Knockdown Reagent:	MO1-msna
Anatomical Term:	intersegmental vessel blood vessel endothelial cell
Stage Range:	Prim-15 to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	is2Tg is2Tg + MO1-msna
Knockdown Reagent:	MO1-msna
Observed In:	blood vessel lumenization intersegmental vessel
Stage Range:	Prim-15 to Long-pec

Phenotype Detail

Acknowledgments

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