ZFIN ID: ZDB-FIG-100506-62
Sheng et al., 2010 - Deletion of the WD40 domain of LRRK2 in Zebrafish causes Parkinsonism-like loss of neurons and locomotive defect. PLoS Genetics   6(4):e1000914 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Protruding-mouth

Fig. 5 WISH Analysis of the loss of TH+ cell in WD40 morphant and its rescue by over-expressing wild-type zLRRK2 or hLRRK2 as well as hLRRK2 mutant alleles.

(A) WISH analysis at 3 dpf shows the decreased TH expression in WD40 deletion morphant as well as the successful rescue by over-expressing either zLRRK2 or hLRRK2. The analysis also shows the decreased TH expression in the fish with over-expression of wild-type zLRRK2 and hLRRK2. This neurodegenerative phenotype could not be rescued by the treatment of L-dopa. (B) Quantification of rescue effect of zLRRK2 or hLRRK2 on TH+ cell loss in WD40 morphant. ***P<0.001 (unpaired Student′s t-test). (C) Quantification of rescue effect of hG2019S or hG2385R on TH+ cell loss in WD40 morphant. **P<0.01, ***P<0.001 (unpaired Student′s t-test).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
th WT standard conditions Protruding-mouth diencephalon dopaminergic neuron ISH
WT + MO1-lrrk2 + MO2-lrrk2 standard conditions Protruding-mouth diencephalon dopaminergic neuron ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-lrrk2 + MO2-lrrk2 standard conditions Protruding-mouth diencephalon dopaminergic neuron decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.