ZFIN ID: ZDB-FIG-100506-59
Sheng et al., 2010 - Deletion of the WD40 domain of LRRK2 in Zebrafish causes Parkinsonism-like loss of neurons and locomotive defect. PLoS Genetics   6(4):e1000914 Full text @ PLoS Genet.
Anatomical Terms:
Stage Range: 1-cell to Adult

Fig. 1 Expression profiling of lrrk2 in zebrafish.

(A) Quantitative RT–PCR analysis of zlrrk2 mRNA expression from 1 cell stage to 10 dpf. (B–D) Northern blot analysis (B), quantitative RT–PCR (C), and Western blot analyses (D) of zLRRK2 expression in gut, muscle, ovary, and brain of adult fish. (E) In situ hybridization of zlrrk2 mRNA at 24 hpf and 6 dpf stage. For 6 dpf stage, cryo-sectioning was performed in the position labeled as 1 and 2.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
lrrk2 WT standard conditions 1-cell to Sphere whole organism RTPCR
Shield to 75%-epiboly Not Detected whole organism RTPCR
Bud to Days 7-13 whole organism RTPCR
Prim-5 brain ISH
Day 6 brain ISH
Ab1-lrrk2 Adult brain WB
Adult brain RTPCR
Adult brain NB
Ab1-lrrk2 Adult gut WB
Adult gut NB
Adult gut RTPCR
Ab1-lrrk2 Adult muscle WB
Adult muscle RTPCR
Adult muscle NB
Ab1-lrrk2 Adult ovary WB
Adult ovary RTPCR
Adult ovary NB
Antibody Labeling Details No data available
Phenotype Details No data available
ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.