ZFIN ID: ZDB-FIG-100506-61
Sheng et al., 2010 - Deletion of the WD40 domain of LRRK2 in Zebrafish causes Parkinsonism-like loss of neurons and locomotive defect. PLoS Genetics   6(4):e1000914 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibody:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage: Day 6
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Day 6

Fig. 4 Analysis by acetylated tubulin staining of embryos at 6 dpf.

Compared to the uninjected embryo, the WD40 deletion morphant has reduced and disorganized axon tracts in the midbrain of zebrafish. This phenotype could be rescued by over-expressing either zLRRK2 (WD40+zLRRK2) or hLRRK2 (WD40+hLRRK2) mRNA. **P<0.01, ***P<0.001 (unpaired Student′s t-test).

Gene Expression Details No data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-tuba IHC WT standard conditions Day 6 brain axon
IHC WT + MO1-lrrk2 + MO2-lrrk2 standard conditions Day 6 brain axon
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-lrrk2 + MO2-lrrk2 standard conditions Day 6 optic tectum axon decreased amount, abnormal
Day 6 optic tectum neuron decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.