ZFIN ID: ZDB-FIG-100506-61
Sheng et al., 2010 - Deletion of the WD40 domain of LRRK2 in Zebrafish causes Parkinsonism-like loss of neurons and locomotive defect. PLoS Genetics   6(4):e1000914 Full text @ PLoS Genet.
Knockdown Reagents:
Anatomical Term:
Stage: Day 6
Knockdown Reagents:
Observed In:
Stage: Day 6

Fig. 4 Analysis by acetylated tubulin staining of embryos at 6 dpf.

Compared to the uninjected embryo, the WD40 deletion morphant has reduced and disorganized axon tracts in the midbrain of zebrafish. This phenotype could be rescued by over-expressing either zLRRK2 (WD40+zLRRK2) or hLRRK2 (WD40+hLRRK2) mRNA. **P<0.01, ***P<0.001 (unpaired Student′s t-test).

Gene Expression Details No data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-tuba IHC WT standard conditions Day 6 brain axon
IHC WT + MO1-lrrk2 + MO2-lrrk2 standard conditions Day 6 brain axon
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-lrrk2 + MO2-lrrk2 standard conditions Day 6 optic tectum axon decreased amount, abnormal
Day 6 optic tectum neuron decreased amount, abnormal
ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.