ZFIN ID: ZDB-FIG-100506-43
Nakano et al., 2010
- Biogenesis of GPI-anchored proteins is essential for surface expression of sodium channels in zebrafish Rohon-Beard neurons to respond to mechanosensory stimulation.
Development (Cambridge, England)
137(10):1689-1698 Full text @ Development
Surface expression of sodium channels is impaired in mutant RB neurons. (A-F) Wholemount immunostaining showed Nav expression in wild-type and mutant RB neurons at 2 dpf. (A) Anti-HuC labeled cell bodies of wild-type RB neurons at the dorsal spinal cord. Dotted lines represent somite boundaries. (B) Immunostaining with anti-pan Nav displayed Nav expression in wild-type spinal cord. (C) A merged image showed that all HuC-positive RB cells expressed Nav in wild-type. (D) Labeling with anti-HuC showed RB neurons in mutant spinal cord. (E) Anti-pan Nav labeled dorsally located spinal neurons in mutants. (F) All of the HuC-positive mutant RB neurons expressed Nav. (G-R) Dissociated RB neurons (GFP-positive) from 2 dpf embryos were labeled with anti-Scn1bb, with or without TX-100, which permeabilizes the plasma membrane. Note that anti-Scn1bb reacts with the extracellular domain of Scn1bb. (G-I) Scn1bb staining in the permeable condition (+TX-100) demonstrated Scn1bb expression in a wild-type RB neuron. (J-L) Labeling of Scn1bb in the impermeable condition (-TX-100) displayed surface distribution of Scn1bb in a wild-type RB cell. (M-O) Scn1bb staining with TX-100 showed Scn1bb expression in a wild-type RB neuron. (P-R) In the absence of TX-100, the surface labeling of Scn1bb was missing in a mutant RB cell, suggesting that Scn1bb exists inside the mutant cell without expression at the surface.
Antibody Labeling Details
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article.
Please note that this material may be protected by copyright.
Full text @ Development