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ZIRC
ZFIN ID: ZDB-FIG-100506-42
Nakano et al., 2010 - Biogenesis of GPI-anchored proteins is essential for surface expression of sodium channels in zebrafish Rohon-Beard neurons to respond to mechanosensory stimulation. Development (Cambridge, England)   137(10):1689-1698 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Anatomical Term:
Stage Range: Long-pec to Day 4
PHENOTYPE:
Fish:
Observed In:
Stage: Day 4

Fig. 2 Programmed cell death of RB neurons is diminished in mi310 mutants. (A) In a lateral view of a wild-type embryo at 2 dpf, anti-HuC labeled large cell bodies of RB neurons (green). Dashed lines represent somite boundaries. (B) A mutant embryo displayed a comparable number of HuC-positive cells at 2 dpf. (C) In wild type, RB neurons were eliminated by 4 dpf. (D) HuC-positive RB cells were observed in mutant larva at 4 dpf. (E) The number of RB neurons is significantly reduced between 2 and 4 dpf in wild type but not in mutants. (F) In a dorsal view of a wild-type embryo, some HuC-positive neurons (magenta) were also positive for TUNEL (green) at 2 dpf. (G) HuC-TUNEL double-positive neurons were rarely observed in mutants.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
elavl3 Ab1-elavl WT standard conditions Long-pec Rohon-Beard neuron cell body IHC
Ab1-elavl Day 4 Rohon-Beard neuron cell body IHC
Ab1-elavl pigumi310/mi310 standard conditions Long-pec Rohon-Beard neuron cell body IHC
Ab1-elavl Day 4 Rohon-Beard neuron cell body IHC
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
pigumi310/mi310 standard conditions Day 4 neuron apoptotic process decreased occurrence, abnormal
Day 4 Rohon-Beard neuron alive, abnormal
Day 4 Rohon-Beard neuron increased age, abnormal
Day 4 Rohon-Beard neuron increased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development