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ZFIN ID: ZDB-FIG-100506-46
Nakano et al., 2010 - Biogenesis of GPI-anchored proteins is essential for surface expression of sodium channels in zebrafish Rohon-Beard neurons to respond to mechanosensory stimulation. Development (Cambridge, England)   137(10):1689-1698 Full text @ Development
Anatomical Term:
Stage Range: 2-cell to 1-4 somites

Fig. 8 pigu is expressed ubiquitously by whole embryos. In situ hybridization with pigu antisense (A-J) or sense probes (K-T). Wholemounts and DIC images at the spinal cord showed that pigu is expressed by whole zebrafish embryos at 1 dpf (A,B) and 2 dpf (C,D). Expression of pigu mRNA was also observed at 0.2 hpf (1-cell stage; E), 0.75 hpf (2-cell stage; F), 1 hpf (4-cell stage; G), 1.25 hpf (8-cell stage; H), 4 hpf (sphere stage; I) and 11 hpf (early segmentation stage; J). Staining with sense probe displayed no pigu expression at any stages (K-T).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
pigu WT standard conditions 2-cell whole organism ISH
1-cell whole organism ISH
4-cell whole organism ISH
8-cell whole organism ISH
Sphere whole organism ISH
1-4 somites whole organism ISH
Antibody Labeling Details No data available
Phenotype Details No data available
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development