ZFIN ID: ZDB-FIG-100415-4
Wang et al., 2009 - Zebrafish beta-adrenergic receptor mRNA expression and control of pigmentation. Gene   446(1):18-27 Full text @ Gene
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 5-9 somites to Adult

Fig. 3 mRNA expression of zebrafish adrb family in developmental stages and adult tissues. Developmental profiles of gene expression are shown on the right (A–E); relative mRNA expression levels in tissues of zebrafish at 6 mpf are shown on the left (F–J). Six individual zebrafish at 6 mpf were used for organ-specific RNA isolation. SYBR Green-based quantitative real-time PCR was used to quantify the expression levels of adrb family. Relative transcript ratios were determined by normalizing threshold cycles (Ct) values to β-actin expression. Data are presented relative to the value in the most highly expressing time point or tissue.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
adrb1 WT standard conditions 5-9 somites to Protruding-mouth whole organism RTPCR
Day 5 to Days 30-44 whole organism RTPCR
Adult adipose tissue RTPCR
Adult brain RTPCR
Adult eye RTPCR
Adult heart RTPCR
Adult liver RTPCR
Adult pancreas RTPCR
Adult testis RTPCR
adrb2a WT standard conditions Day 5 to Days 30-44 whole organism RTPCR
Adult adipose tissue RTPCR
Adult blood RTPCR
Adult brain RTPCR
Adult eye RTPCR
Adult gill RTPCR
Adult gut RTPCR
Adult heart RTPCR
Adult integument RTPCR
Adult kidney RTPCR
Adult liver RTPCR
Adult pancreas RTPCR
Adult spleen RTPCR
Adult testis RTPCR
Adult trunk musculature RTPCR
adrb2b WT standard conditions 14-19 somites to Protruding-mouth whole organism RTPCR
Day 5 to Days 30-44 whole organism RTPCR
Adult liver RTPCR
Adult pancreas RTPCR
Adult trunk musculature RTPCR
adrb3a WT standard conditions Prim-5 to Protruding-mouth whole organism RTPCR
Day 5 to Days 30-44 whole organism RTPCR
Adult brain RTPCR
adrb3b WT standard conditions Day 5 to Days 7-13 whole organism RTPCR
Days 14-20 Not Detected whole organism RTPCR
Days 30-44 whole organism RTPCR
Adult brain RTPCR
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Gene for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Gene, 446(1), Wang, Z., Nishimura, Y., Shimada, Y., Umemoto, N., Hirano, M., Zang, L., Oka, T., Sakamoto, C., Kuroyanagi, J., and Tanaka, T., Zebrafish beta-adrenergic receptor mRNA expression and control of pigmentation, 18-27, Copyright (2009) with permission from Elsevier. Full text @ Gene