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Fig. 10

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ZDB-FIG-100408-13

Publication

Veldman et al., 2010 - Tuba1a gene expression is regulated by KLF6/7 and is necessary for CNS development and regeneration in zebrafish

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Fig. 10

Regeneration-dependent induction of KLF6a and 7a is necessary for tuba1a and p27 gene induction following optic nerve injury. The optic nerve of adult tuba1a:GFP transgenic fish was lesioned and KLF6a/ KLF7a targeting MO or a control MO was applied to the optic nerve stump as described in Experimental methods. 3 days later retinas were harvested and RNA purified for use in RT-PCR reactions. Shown is an ethidium bromide stained gel showing induction of GFP, tuba1a, p27 and c-jun following optic nerve injury. GAPDH is used as a loading control. Note that KLF6a and 7a knockdown (KLF6/7 MO) caused a significant reduction in GFP, tuba1a and p27 gene induction following optic nerve injury.

Expression Data

Genes:	cdkn1bb EGFP jun tuba1a
Fish:	mi2Tg mi2Tg + MO1-klf6a + MO1-klf7b + MO2-klf6a + MO2-klf7b
Condition:	surgical manipulation: cranial nerve II
Knockdown Reagents:	MO1-klf6a MO1-klf7b MO2-klf6a MO2-klf7b
Anatomical Term:	retina
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Molecular and cellular neurosciences, 43(4), Veldman, M.B., Bemben, M.A., and Goldman, D., Tuba1a gene expression is regulated by KLF6/7 and is necessary for CNS development and regeneration in zebrafish, 370-383, Copyright (2010) with permission from Elsevier. Full text @ Mol. Cell Neurosci.