|ZFIN ID: ZDB-FIG-100401-11|
Fig. 5 Endothelial and vascular expression of syk rescues angioblasts migration in vegfa morphants. (A–B) Compared to 30 hpf wildtype Tg(fli1:egfp)y1 embryos with complete ISVs (A), vegfa morphants lack any ISV sprouting (B). (C–D) Expression of a C-terminal tagged syk under the control of a vascular fli1 promoter (fli1:syk:myc, C) or a blood gata1 promoter (gata1:syk:myc, D) in single celled embryos injected with 4 ng of vegfa morpholino results in increased number of ISV sprouting (white arrow). Expression of the fli1:syk:myc construct is detected by immunohistochemistry against the myc tag (red, C, D). The large myc-expressing cells visible in the dorsal and mid-trunk of gata:syk injected embryos are superficial and do not represent expression of the transgene in blood vessels, while ventral myc expression cells are within the axial vessels.
|y1Tg + MO2-vegfaa||standard conditions||Prim-15||intersegmental vessel aplastic, abnormal|
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Reprinted from Developmental Biology, 340(1), Christie, T.L., Carter, A., Rollins, E.L., and Childs, S.J., Syk and Zap-70 function redundantly to promote angioblast migration, 22-29, Copyright (2010) with permission from Elsevier. Full text @ Dev. Biol.