FIGURE

Fig. 2

ID

ZDB-FIG-100401-5

Publication

Christie et al., 2010 - Syk and Zap-70 function redundantly to promote angioblast migration

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Fig. 2

syk^LO and zap-70^LO morphants display late stage ISV patterning defects. (A) The trunk of 5 dpf wild-type Tg(fli1a:EGFP)^y1 embryos display regularly patterned ISVs between somites, with the dorsal-most ISV endothelial cell forming a clear T shape and contributing to the DLAV. In contrast, 5 dpf syk^LO (B), zap-70^LO (C), and syk^LO:zap-70^LO (D) morphants display abnormal ISV vasculature with mild patterning defects. The ventral stalk ISV endothelial cell is relatively normal, and the dorsal T-cell still contributes to the DLAV, but the branching of the cell into the T shape is abnormal (asterisks) and can occur as ventrally as midsomite (arrows). These dorsal patterning defects are more pronounced in the syk^LO:zap-70^LO morphants.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	y1Tg + MO1-syk y1Tg + MO2-zap70 y1Tg + MO1-syk + MO2-zap70
Knockdown Reagents:	MO1-syk MO2-zap70
Observed In:	dorsal longitudinal anastomotic vessel intersegmental vessel
Stage:	Day 5

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 340(1), Christie, T.L., Carter, A., Rollins, E.L., and Childs, S.J., Syk and Zap-70 function redundantly to promote angioblast migration, 22-29, Copyright (2010) with permission from Elsevier. Full text @ Dev. Biol.