FIGURE

Fig. 4

ID

ZDB-FIG-100401-10

Publication

Christie et al., 2010 - Syk and Zap-70 function redundantly to promote angioblast migration

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Fig. 4

syk rescues angioblast migration defects after syk knockdown. (A–D) syk and zap-70 are expressed in a ubiquitous manner and have overlapping expression profiles. At 16 somites (16s) syk (A) and zap-70 (C) are highly expressed in the head, with low levels of expression throughout the rest of the embryo. (B, D) By 21s increased expression is seen in the ventral trunk. (E–F) By 30 hpf, the ISVs of wildtype Tg(fli1:egfp)^y1 embryos have reached the dorsal portion of the embryo to form the DLAV (E) while the ISVs of syk morphants are delayed at the mid-trunk (F). (G–H) Expression of a C-terminal myc-tagged syk in the vasculature (G, fli1:syk:myc) or blood (H, gata1:syk:myc) partially rescues the number of ISVs that have reached the DLAV (black arrow).

Expression Data

Genes:	syk zap70
Fish:	WT
Anatomical Term:	whole organism
Stage Range:	14-19 somites to 20-25 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	y1Tg + MO1-syk
Knockdown Reagent:	MO1-syk
Observed In:	intersegmental vessel
Stage:	Prim-15

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 340(1), Christie, T.L., Carter, A., Rollins, E.L., and Childs, S.J., Syk and Zap-70 function redundantly to promote angioblast migration, 22-29, Copyright (2010) with permission from Elsevier. Full text @ Dev. Biol.