CETs that define the epicardium in vivo. A, B: ET27 with EGFP-positive cells covering the ventricle; note that EGFP expression in the BA precedes that in the epicardium; C, D: The EGFP-positive epicardial cells (arrow) enveloping the internal cmlc2 highlighted myocardium at high magnification. E: Cross-section of the adult ET27 heart at low magnification, with GFP-labelled epicardium on the external surface of the BA, atrium and ventricle (arrow). B-D: Double transgenic embryos of ET27 and Tg(cmlc2:dsRed). All except D are fluorescent images. D: Composite fluorescent/DIC images. A, atrium; BA, bulbus arteriosus; V, ventricle.
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