ZFIN ID: ZDB-FIG-100309-51
Poon et al., 2010 - Zebrafish cardiac enhancer trap lines: New tools for in vivo studies of cardiovascular development and disease. Developmental dynamics : an official publication of the American Association of Anatomists   239(3):914-926 Full text @ Dev. Dyn.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Anatomical Terms:
Stage Range: Protruding-mouth to Adult

Fig. 6 Developmental changes in CET lines with early BA-specific expression. A: EGFP expression in the BA and ventral aorta (VA) in wildtype 3-dpf ET27. B: smo mutation affects the ventral aorta, but not the domain of EGFP expression corresponding to BA. C, C′: ET27 adult heart sections shown at low (C) and high (C′) magnification. D: ET31 embryonic expression in BA. E, E′, E″: Cardiac valves; VB (E′) and AV (E″) marked in adult heart of ET31 shown on F-actin-stained sections. F: BA restricted embryonic expression of GW2C. G: GW2C adult heart sections at low magnification. G′, H: Serial sections of VB valve showing its different morphology at high magnification. I, I′: ET33-1A adult heart sections shown at low (I) and high (I″) magnification, for comparison of endocardal cell morphology in BA with ET27 (C′). A, B, D, F: Ventral view at 3dpf; C, C′: Sections of the adult heart immunostained for EGFP and MF20. E′, E″, H, I: Sections counterstained with F-actin. A-V, atrio-ventricular valve; BA, bulbus arteriosus; VB, ventriculo-bulbar valve; V, ventricle.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP smounspecified/unspecified; sqet27Et standard conditions Protruding-mouth bulbus arteriosus IFL
sqet27Et standard conditions Protruding-mouth bulbus arteriosus IFL
Protruding-mouth ventral aorta IFL
Ab12-GFP Adult bulbus arteriosus IHC
sqet31Et standard conditions Protruding-mouth bulbus arteriosus IFL
Ab12-GFP Adult atrioventricular valve IHC
Ab12-GFP Adult ventriculo bulbo valve IHC
sqgw2CEt standard conditions Protruding-mouth bulbus arteriosus IFL
Ab12-GFP Adult ventriculo bulbo valve IHC
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental dynamics : an official publication of the American Association of Anatomists for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dev. Dyn.