ZFIN ID: ZDB-FIG-091215-65
Lee et al., 2009 - Notch Signaling Functions as a Cell-Fate Switch between the Endothelial and Hematopoietic Lineages. Current biology : CB   19(19):1616-1622 Full text @ Curr. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Conditions:
Anatomical Term:
Stage: 14-19 somites

Fig. 2 Notch Signaling Positively Regulates the Number of Hematopoietic Cells during Zebrafish Development

(A) Whole-mount RNA in situ hybridization showing the expression of gata1 in 18 hpf DMSO-treated or DAPT-treated embryos. Two additional markers, krox20 and otx1, which label the hindbrain and neuroectoderm, respectively, were used as positive controls. Notice the reduced expression of gata1 in DAPT-treated embryos (black arrow).

(B) Transverse sections of 18 hpf DMSO- or DAPT-treated embryos visualized for TOPRO (white), gata1:GFP (green), and phalloidin (red).

(C) Quantification of the hematopoietic nuclei per focal plane in DMSO-treated (n = 27) or DAPT-treated (n = 19) embryos.

(D) Transverse sections of 18 hpf phenotypic wild-type siblings and Tg(hsp70l:Gal4)kca4;Tg(UAS:myc-NICD)kca3 embryos visualized for TOPRO (white), gata1:GFP (green), and c-Myc as a surrogate measure for NICD expression (red).

(E) Quantification of the hematopoietic nuclei per focal plane in phenotypic wild-type siblings (n = 28) and NICD-overexpressing embryos (n = 23). DAPT-treated embryos contained 4.89 (s = 2.15) erythrocyte nuclei, in comparison to 8.07 (s = 2.38) nuclei in DMSO-treated embryos (ANOVA, p < 10-4). Conversely, embryos overexpressing the NICD contained 9.89 (s = 2.53) erythrocyte nuclei, in comparison to 6.72 (s = 1.43) nuclei per section in phenotypic wild-type siblings (ANOVA, p < 10-6), indicating that Notch signaling positively modulates the number of hematopoietic cells.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP kca3Tg; kca4Tg; la781Tg control 14-19 somites nucleate erythrocyte IFL
kca3Tg; kca4Tg; la781Tg heat shock 14-19 somites nucleate erythrocyte IFL
GFP la781Tg control 14-19 somites nucleate erythrocyte IFL
la781Tg chemical treatment: DAPT 14-19 somites nucleate erythrocyte IFL
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Current biology : CB for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Curr. Biol.