IMAGE

Fig. 2

ID
ZDB-IMAGE-091215-63
Genes
Source
Figures for Lee et al., 2009
Image
Figure Caption

Fig. 2 Notch Signaling Positively Regulates the Number of Hematopoietic Cells during Zebrafish Development

(A) Whole-mount RNA in situ hybridization showing the expression of gata1 in 18 hpf DMSO-treated or DAPT-treated embryos. Two additional markers, krox20 and otx1, which label the hindbrain and neuroectoderm, respectively, were used as positive controls. Notice the reduced expression of gata1 in DAPT-treated embryos (black arrow).

(B) Transverse sections of 18 hpf DMSO- or DAPT-treated embryos visualized for TOPRO (white), gata1:GFP (green), and phalloidin (red).

(C) Quantification of the hematopoietic nuclei per focal plane in DMSO-treated (n = 27) or DAPT-treated (n = 19) embryos.

(D) Transverse sections of 18 hpf phenotypic wild-type siblings and Tg(hsp70l:Gal4)kca4;Tg(UAS:myc-NICD)kca3 embryos visualized for TOPRO (white), gata1:GFP (green), and c-Myc as a surrogate measure for NICD expression (red).

(E) Quantification of the hematopoietic nuclei per focal plane in phenotypic wild-type siblings (n = 28) and NICD-overexpressing embryos (n = 23). DAPT-treated embryos contained 4.89 (s = 2.15) erythrocyte nuclei, in comparison to 8.07 (s = 2.38) nuclei in DMSO-treated embryos (ANOVA, p < 10-4). Conversely, embryos overexpressing the NICD contained 9.89 (s = 2.53) erythrocyte nuclei, in comparison to 6.72 (s = 1.43) nuclei per section in phenotypic wild-type siblings (ANOVA, p < 10-6), indicating that Notch signaling positively modulates the number of hematopoietic cells.

Figure Data
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Curr. Biol.