ZFIN ID: ZDB-FIG-091215-64
Lee et al., 2009 - Notch Signaling Functions as a Cell-Fate Switch between the Endothelial and Hematopoietic Lineages. Current biology : CB   19(19):1616-1622 Full text @ Curr. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Conditions:
Anatomical Term:
Stage: 14-19 somites

Fig. 1 Notch Signaling Negatively Regulates the Number of Endothelial Cells during Zebrafish Development

(A) Transverse sections of 18 hpf embryos, DMSO- or DAPT-treated, visualized for TOPRO (white), kdrl:GFP (green), and phalloidin (red).

(B) Quantification of endothelial nuclei per focal plane in DMSO-treated (n = 30) or DAPT-treated (n = 44) embryos.

(C) Transverse sections of 18 hpf phenotypic wild-type siblings and Tg(hsp70l:Gal4)kca4;Tg(UAS:myc-NICD)kca3 embryos visualized for TOPRO (white), kdrl:GFP (green), and c-Myc as a surrogate measure for NICD expression (red).

(D) Quantification of endothelial nuclei per focal plane in phenotypic wild-type (n = 31) and NICD-overexpressing (n = 28) embryos. Embryos treated with DAPT contained 6.66 (s = 2.42) endothelial nuclei per section, whereas those treated with DMSO had 4.03 (s = 1.27) endothelial nuclei (ANOVA, p < 10-6). Conversely, embryos overexpressing the NICD contained 3.14 (s = 0.80) endothelial nuclei, in comparison to 4.42 (s = 1.09) nuclei per section in phenotypic wild-type siblings (ANOVA, p < 10-5), suggesting that Notch signaling negatively regulates endothelial cell number.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP kca3Tg; kca4Tg; s843Tg control 14-19 somites endothelial cell IFL
kca3Tg; kca4Tg; s843Tg heat shock 14-19 somites endothelial cell IFL
s843Tg control 14-19 somites endothelial cell IFL
s843Tg chemical treatment: DAPT 14-19 somites endothelial cell IFL
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Current biology : CB for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Curr. Biol.