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ZFIN ID: ZDB-FIG-090904-67
Arrington et al., 2009 - Extra-embryonic syndecan 2 regulates organ primordia migration and fibrillogenesis throughout the zebrafish embryo. Development (Cambridge, England)   136(18):3143-3152 Full text @ Development
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: 20-25 somites

Fig. 4 Extra-embryonic Sdc2, but not embryonic Sdc2, is required for fibronectin fibrillogenesis throughout the embryo. (A) Diagram of an embryo cut in cross-section at the level of the cardiac primordia. Fibronectin fibrils form in the extracellular space adjacent to the cardiac primordia and surrounding the neural tube. (B-F) Transverse sections (right) of 22-somite embryos immunostained for fibronectin (green). Circular diagrams (left) represent different injection patterns of sdc2 morpholino (red) and sdc2 mRNA (blue). (B) Uninjected wild-type embryos (16/16 embryos) and (C) traditional sdc2 morphants (4/4 embryos) had normal fibrillogenesis, indicating that embryonic Sdc2 is not required for fibrillogenesis. (D) yolksdc2MO morphants had no fibril formation in 8/15 embryos. With co-injection of sdc2 mRNA into the yolk at the dome stage, fibrillogenesis was rescued in 17/20 embryos (E). By contrast, injection of sdc2 mRNA at the 1-to-2-cell stage, to overexpress Sdc2 in embryonic cells, followed by injection of sdc2 morpholino into yolk at the dome stage (F), did not rescue fibrillogenesis (7/10 embryos). As noted by the numbers adjacent to the confocal microscopy images, a higher percentage of normal cardiac migration correlates with the presence of fibronectin fibrils.

Gene Expression DetailsNo data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO4-sdc2 standard conditions 20-25 somites supramolecular fiber organization disrupted, abnormal
AB + MO5-sdc2 standard conditions 20-25 somites supramolecular fiber organization disrupted, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development