PUBLICATION

Extra-embryonic syndecan 2 regulates organ primordia migration and fibrillogenesis throughout the zebrafish embryo

Authors

Arrington, C.B., and Yost, H.J.

ID

ZDB-PUB-090828-15

Date

2009

Source

Development (Cambridge, England) 136(18): 3143-3152 (Journal)

Registered Authors

Yost, H. Joseph

Keywords

Cardiac, Fibronectin, Gut, Heart, Proteoglycan, Syndecan, Zebrafish

MeSH Terms

Endoderm/cytology
Endoderm/metabolism
Animals
Egg Yolk/metabolism
Zebrafish*/anatomy & histology
Zebrafish*/embryology
Endocardium/cytology
Endocardium/metabolism
Myocytes, Cardiac/cytology
Myocytes, Cardiac/metabolism
Cell Movement/physiology*
Syndecan-2/genetics
Syndecan-2/metabolism*
Cell Polarity
Cell Lineage
Zebrafish Proteins/genetics
Zebrafish Proteins/metabolism*
Embryo, Nonmammalian/anatomy & histology
Embryo, Nonmammalian/physiology
Gene Knockdown Techniques
Membrane Proteins/genetics
Membrane Proteins/metabolism
Morphogenesis/physiology*

(all 23)

PubMed

19700618 Full text @ Development

Abstract

One of the first steps in zebrafish heart and gut organogenesis is the migration of bilateral primordia to the midline to form cardiac and gut tubes. The mechanisms that regulate this process are poorly understood. Here we show that the proteoglycan syndecan 2 (Sdc2) expressed in the extra-embryonic yolk syncytial layer (YSL) acts locally at the YSL-embryo interface to direct organ primordia migration, and is required for fibronectin and laminin matrix assembly throughout the embryo. Surprisingly, neither endogenous nor exogenous sdc2 expressed in embryonic cells can compensate for knockdown of sdc2 in the YSL, indicating that Sdc2 expressed in extra-embryonic tissues is functionally distinct from Sdc2 in embryonic cells. The effects of sdc2 knockdown in the YSL can be rescued by extra-embryonic Sdc2 lacking an extracellular proteolytic cleavage (shedding) site, but not by extra-embryonic Sdc2 lacking extracellular glycosaminoglycan (GAG) addition sites, suggesting that distinct GAG chains on extra-embryonic Sdc2 regulate extracellular matrix assembly, cell migration and epithelial morphogenesis of multiple organ systems throughout the embryo.

Genes / Markers

Figures

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Expression

Fish	Conditions	Stage	Anatomy	Assay	Figure
twu34Tg	standard conditions	20-25 somites	heart primordium	IHC	Fig. 3
twu34Tg + MO4-sdc2	standard conditions	20-25 somites	heart primordium	IHC	Fig. 3
AB	standard conditions	10-13 somites	unspecified	ISH	Fig. 5
AB + MO4-sdc2	standard conditions	10-13 somites	unspecified	ISH	Fig. 5
AB + MO4-sdc2	standard conditions	Prim-25	gut	ISH	Fig. 2
AB + MO5-sdc2	standard conditions	Prim-25	gut	ISH	Fig. 2
AB	standard conditions	10-13 somites	unspecified	ISH	Fig. 5
AB + MO4-sdc2	standard conditions	10-13 somites	unspecified	ISH	Fig. 5
AB	standard conditions	10-13 somites	unspecified	ISH	Fig. 5
AB + MO4-sdc2	standard conditions	10-13 somites	unspecified	ISH	Fig. 5

1 - 10 of 17

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Phenotype

Fish	Conditions	Stage	Phenotype	Figure
AB + MO4-sdc2	standard conditions	14-19 somites	whole organism morphology, normal	Fig. 2
AB + MO4-sdc2	standard conditions	20-25 somites	basement membrane organization disrupted, abnormal	Fig. 3
AB + MO4-sdc2	standard conditions	20-25 somites	supramolecular fiber organization disrupted, abnormal	Fig. 4
AB + MO4-sdc2	standard conditions	26+ somites	cell migration involved in heart development disrupted, abnormal	Fig. 2
AB + MO4-sdc2	standard conditions	Prim-25	digestive tract development disrupted, abnormal	Fig. 2
AB + MO4-sdc2	standard conditions	Prim-25	extension morphology, abnormal	Fig. 2
AB + MO4-sdc2	standard conditions	Prim-25	heart development disrupted, abnormal	Fig. 2
AB + MO4-sdc2	standard conditions	Prim-25	pericardium edematous, abnormal	Fig. 2
AB + MO4-sdc2	standard conditions	Prim-25	pigmentation decreased occurrence, abnormal	Fig. 2
AB + MO4-sdc2	standard conditions	Prim-25	whole organism decreased size, abnormal	Fig. 2

1 - 10 of 26

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Mutations / Transgenics

Allele	Construct	Type	Affected Genomic Region
twu34Tg	Tg(myl7:EGFP)	Transgenic Insertion
y1Tg	Tg(fli1:EGFP)	Transgenic Insertion

Human Disease / Model

Sequence Targeting Reagents

Target	Reagent	Reagent Type
sdc2	MO4-sdc2	MRPHLNO
sdc2	MO5-sdc2	MRPHLNO

Fish

Fish
AB
AB + MO4-sdc2
AB + MO5-sdc2
twu34Tg
twu34Tg + MO4-sdc2
twu34Tg + MO5-sdc2
y1Tg

Antibodies

Orthology

Engineered Foreign Genes

Marker	Marker Type	Name
EGFP	EFG	EGFP

Mapping

Arrington et al., 2009 ZDB-PUB-090828-15 PMID:19700618

Extra-embryonic syndecan 2 regulates organ primordia migration and fibrillogenesis throughout the zebrafish embryo

Arrington et al., 2009

ZDB-PUB-090828-15
PMID:19700618