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ZIRC
ZFIN ID: ZDB-FIG-090904-66
Arrington et al., 2009 - Extra-embryonic syndecan 2 regulates organ primordia migration and fibrillogenesis throughout the zebrafish embryo. Development (Cambridge, England)   136(18):3143-3152 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibodies:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: 20-25 somites
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: 20-25 somites

Fig. 3 Basement membrane and epithelial cell polarity are disrupted in yolksdc2MO embryos. (A,B) Transverse sections of wild-type (A) and yolksdc2MO (B) embryos at 22 somites reveal laminin deposition (white arrow) between the cardiac and ventral foregut primordia in wild-type embryos (3/3) and reduced laminin in yolksdc2MO embryos (5/6). Laminin, red; β-catenin, green. (C-F) Transverse sections of Tg(cmlc2:GFP) embryos at 22 somites immunostained for the cell-junction protein aPKC (green) and cardiac primordia (red). Magnified views of cardiac precursors (marked by rectangles in C and D) reveal apicolateral localization of aPKC protein (arrows) in noninjected embryos (E) and diminished aPKC in yolksdc2MO embryos (F).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP AB9-GFP twu34Tg standard conditions 20-25 somites heart primordium IHC
AB9-GFP twu34Tg + MO4-sdc2 standard conditions 20-25 somites heart primordium IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-prkcz IHC twu34Tg standard conditions 20-25 somites gut endothelial cell apicolateral plasma membrane
IHC 20-25 somites heart primordium apicolateral plasma membrane
IHC twu34Tg + MO4-sdc2 standard conditions 20-25 somites gut endothelial cell apicolateral plasma membrane
IHC 20-25 somites heart primordium apicolateral plasma membrane
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO4-sdc2 standard conditions 20-25 somites basement membrane organization disrupted, abnormal
AB + MO5-sdc2 standard conditions 20-25 somites basement membrane organization disrupted, abnormal
twu34Tg + MO4-sdc2 standard conditions 20-25 somites apical protein localization disrupted, abnormal
20-25 somites basement membrane organization disrupted, abnormal
twu34Tg + MO5-sdc2 standard conditions 20-25 somites apical protein localization disrupted, abnormal
20-25 somites basement membrane organization disrupted, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development